Variations of contents of endogenous hormones, namely Gibberellin (GA3), Indoleacetic Acid (IAA), Abscisic Acid(ABA) and Zeatin (ZT), in scales, bulblets and new roots of Lilium oriental Sorbonne' during scale propagation process were analyzed using high-performance liquid chromatography. Results show that the four endogenous hormones have distinct distribution in scales, bulblets and new roots during bulblet formation and growth process: ZT and GA3 concentrations are higher in scales than in bulblets and new roots; IAA concentration is higher in new roots than in scales and bulblets; ABA concentration is the highest in bulblets. All the four hormones have increasing trends in scales and bulblets with different increase rate during scale propagation. The net increasing rate of ZT concentration in scales is 109%, which is the highest among the four hormones. IAA concentration has a net increasing rate of 102% in bulblets. The hormones except ABA are in increasing trends in new roots, and the increasing rate of ZT concentration is the highest, by 493%.