WANG Honglun and his teams isolated and purified flavonoids and polyhydroxystilbenes from Trigonella foenum-graecum L. seed extracts using high-speed counter-current chromatography (HSCCC) in two steps with two different kinds of solvent systems.

In the first step, n-hexane:ethyl acetate:methanol:water (1:5:1:5, v/v) was used as the twophase solvent system, desoxy-rhaponticin and rhapontigenin were purified, and orientin, vitexin, isovitexin, and rhaponticin were eluted together.

In the second step, ethyl acetate:water (1:1, v/v) was used as the two-phase solvent system, and orientin, vitexin, rhaponticin, and isovitexin were isolated and purified. Purities of the isolated compounds were determined using HPLC, while UV spectra, ¹H-NMR, and ¹³C-NMR aided in structure identification.

FIG. HPLC chromatograms of Trigonella foenum-graecum L. samples and HSCCC peak fractions. HPLC chromatograms for sample 1 (a) and sample 2 (b). 

Additional Information:

1 Author Information: Yanfeng He, Huanhuan Lv, Xiaoyan Wang, Yourui Suo,and Honglun Wang

Correspondence: hlwang@nwipb.cas.cn

2 Published: Separation Science and Technology,2014, 49: 580–587